Nocardioides perillae sp. nov., isolated from surface-sterilized roots of Perilla frutescens.
Source
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Abstract
A Gram-stain-positive, rod-shaped actinobacterium, designated strain I10A-01402(T), was isolated from surface-sterilized roots of a medicinal plant, Perilla frutescens, collected in a suburb of Beijing, China. Chemotaxonomically, the strain contained ll-diaminopimelic acid as the diagnostic diamino acid and MK-8(H4) as the predominant menaquinone. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The major fatty acids were C17?:?1ω9c, C18?:?1ω9c, C17?:?0, C16?:?0 and iso-C16?:?0. The genomic DNA G+C content was 70.4 mol%. 16S rRNA gene sequence analysis indicated that strain I10A-01402(T) belonged to the genus Nocardioides. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate formed a robust cluster with Nocardioides ginsengisegetis Gsoil 485(T), N. koreensis MSL-09(T) and N. alkalitolerans KSL-1(T). On the basis of the evidence from our polyphasic taxonomic study, a novel species, Nocardioides perillae sp. nov., is proposed. The type strain is I10A-01402(T) (?=?CPCC 203382(T) ?=?DSM 24552(T) ?=?KCTC 29022(T)).